| Product Name |
CONFOCHECK – Complete Protein Analysis Solution |
| Main Applications |
Detection and quantification of conformational changes in proteins, Protein concentration determination |
| Speed |
Quantitative determination of protein concentration and secondary structure elements (alpha-helix, beta-sheet) accomplished within seconds via internal calibrations |
| Sample Handling |
Equipped with several specific protein sampling cuvettes, Allows measurement of solved and membrane bound proteins, Works even at very low amounts (10 µg), Temperature-controlled sample cuvettes by thermostat for optimal temperature constancy |
| FT-IR Protein Applications |
Protein quantification, Detection of conformational changes, Protein dynamics (temperature induced conformational changes), Monitoring conformational changes during protein aggregation, precipitation and crystallization, Determination of secondary structure, Quantification of all solutes in aqueous samples (buffers, excipients, detergents) |
| Pharma Applications |
Pre-formulation and formulation development: Analysis of formulation influence on protein stability, Stability studies (stress tests): Influence of pH, temperature, mutation on protein stability, Quality control: Check protein concentration, structure, and correct product composition (buffer, excipients) in production environment, Binding studies: Analysis of ligand-binding impact on protein structure |
| CONFOCHECK Benefits |
Complete system can be validated for pharmaceutical quality control use, Optimized long-life components minimize maintenance and prolong revalidation cycles, Gold optics for maximum sensitivity in MIR, 10 year warranty on laser, 10 year warranty on interferometer, 5 year warranty on MIR source |
| AquaSpec™ Transmission Cell |
Used for measurement of water soluble proteins or solubilized membrane-bound proteins, Flow-through transmission cell with optimal path length of ~7 µm, Permits higher sample throughput and accuracy, Easily measures low protein concentrations (below 0.1 mg/ml), Detects smallest conformational changes, Highest sensitivity for detection of conformational changes, Best accuracy in protein concentration determination |
| AquaSpec™ Applications |
Determination of changes in protein structure, Protein concentration determination, Quality control of biopharmaceuticals, Secondary structure determination, Quantification of various substances in aqueous solutions |
| BioATR II |
Micro-ATR unit specially developed for investigation of temperature-induced unfolding, refolding and denaturation processes, Applied for water solved and membrane-standing proteins, Number of internal reflections in ATR crystal (silicon) adjusted for optimal protein signal in aqueous samples |
| BioATR II Applications |
Automated temperature ramps (0-95°C), Analysis of temperature-induced conformational changes, Measurement of phase transitions in lipids, Quality control of biopharmaceuticals, Membrane-standing proteins, Conformational changes induced by ligand-binding, pH-changes, salts |
| BioATR II Features |
Temperature-controlled by same thermostat as other CONFOCHECK accessories, Automatic temperature ramps from 0 to 95°C to analyze temperature-induced structural changes and protein melting point, Characterizes fibrillation and aggregation processes, Monitors protein structural changes dependent on pH-changes or ligand-binding interactions, Special dialysis top section allows low molecular weight substances to be dialyzed into protein compartment without diluting protein |
| Measurement Time |
Approximately 30 seconds per measurement with direct determination of secondary structure and concentration |
